Serine integrases are emerging as one of the most powerful biological tools for biotechnology. Over the past decade, many research papers have been published on the use of serine integrases in synthetic biology. In this review, we aim to systematically summarize the various studies ranging from structure and the catalytic mechanism to genetic design and interdisciplinary applications. First, we introduce the classification, structure, and catalytic model of serine integrases. Second, we present a timeline with milestones that describes the representative achievements. Then, we summarize the applications of serine integrases in genome engineering, genetic design, and DNA assembly. Finally, we discuss the potential of serine integrases for advancing interdisciplinary research. We anticipate that serine integrases will be further expanded as a versatile genetic toolbox for synthetic biology applications.

Frontiers Translating New Synthetic Biology Advances for

Synthetic Biology: The $3.6 Trillion Science Changing Life as We

2023 Synthetic Biology: Engineering, Evolution & Design (SEED)

SynBio, Free Full-Text

Synthetic Biology: From Promise to Profits

The Synthetic Biology Open Language

Sustainable manufacturing with synthetic biology

Cell-Free Reagents

Explainer: How Synthetic Biology is Redesigning Life

Synthetic Biology US 2024